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Fig. 3 | Plant Methods

Fig. 3

From: RNA-binding protein immunoprecipitation as a tool to investigate plant miRNA processing interference by regulatory proteins of diverse origin

Fig. 3

RIP analysis of precursors associated with 24K or 54K GFP-fused proteins from Citrus psorosis virus (CPsV) in Nicotiana benthamiana. RT-qPCR was performed to determine the accumulation levels of N. benthamiana pre-miR156a (a) or pre-miR171a (b). Fold enrichment of the immunoprecipitated (RIP) precursors was calculated as 2(−ΔΔCt [RIP/background]). Mean values and standard errors of three independent experiments are shown. Statistical analysis was performed using a two-tailed paired t test; * and ** indicate significant differences from RFP control sample at P < 0.05 and P < 0.01 values, respectively. GFP green fluorescent protein, RFP red fluorescent protein

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