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Table 1 Read mapping and gene detection statistics based on starting RNA quantity

From: Combining laser-assisted microdissection (LAM) and RNA-seq allows to perform a comprehensive transcriptomic analysis of epidermal cells of Arabidopsis embryo

RNA template 5 ng 100 pg 75 pg 50 pg 25 pg 10 pg
Librairy size (million) 53.341628 38.317268 25.174298 31.510988 32.682558 29.791112
Mapped reads (million) (percentage) 51.127950 (96%) 30.780261 (81%) 22.402608 (89%) 28.652941 (91%) 29.123427 (89%) 26.701774 (90%)
Detected genes (level 0) 17446 19266 19539 18485 16829 15936
Common reads with 5 ng reference sample (percentage)   15704 (90%) 15880 (98.8%) 15317 (87.8%) 14312 (85%) 13748 (86.3%)
  1. Both first lines are the number of sequenced PE reads in million(s), and number of PE reads kept after mapping and gene association. Detected genes are the number of genes with a1 PE or more. The last line is the number of genes in common between 17446 genes detected with 5 ng compared to the other samples. The raw counts are already rid of rRNA reads, 3–6% have been removed for each sample