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Table 1 Voucher information and differences in the resolution of leaf veins as assessed from vein density (VLA) calculations based on two different 2D X-ray image plate resolutions, the industrial standard (25 µm) compared with our new project standard (7 µm) as well as micro CT (CT)

From: Improved non-destructive 2D and 3D X-ray imaging of leaf venation

Taxon

Collector and collection no. (herbarium acronym)

2D X-ray: 25 µm versus 7 µm

CT versus 2D X-ray (7 µm)

CT image with full vein resolution

Mean VLA (mm mm−2) 25/7 µm

% similarity in VLA calculations

Mean VLA (mm mm−2) 7 µm/CT

% similarity in VLA calculations

Anemia abbottii Maxon

Bisse 27767 (FR)

1.98/1.98

100.0

1.98/1.98

100.0

Yes

Bobgunnia madagascariensis (Desv.) J.H. Kirkbr. and Wiersema

Höhn 19 (FR)

5.26/6.85

76.8***

6.85/11.5

59.6***

Yes

Boscia senegalensis Lam.

Kahlheber 497 (FR)

3.99/4.43

90.1

4.43/4.93

89.9

Yesb

Burkea africana Hook.

Zwarg 81 (FR)

6.35/7.69

82.6***

7.07/8.16

86.7***

Yes

Daniellia oliveri (Rolfe) Hutch. and Dalziel

Kéré 822 (FR)

2.88/3.41

84.5*

3.41/8.31

41.1***

Yesb

Detarium microcarpum Guill. and Perr.

Küppers 636 (FR)

4.02/5.20

77.3***

5.20/6.66

78.1***

Yes

Euthemis leucocarpa Jack

Fuchs 21192 (L)

1.64/1.68

97.6

1.68/7.27

23.1***

Yes

Froesia venezuelensis Steyerm. and G.S. Bunting

Schneider 1 (FR)

n.d.a

n.d.a

n.d.a

n.d.a

Yes

Idertia axillaris (Oliv.) Farron

Bissiengou 1275 (WAG)

3.48/4.20

82.8*

4.21/8.79

47.9***

Yes

Lophira lanceolata Tiegh. ex Keay

Neumann 1530 (FR)

2.41/2.75

87.6*

2.75/4.76

57.8***

Yesb

Mirabilis prostrata (Ruiz and Pav.) Heimerl

Schneider 2842 (FR)

0.87/0.87

100.0

0.87/1.35

64.4***

Noc

Ochna afzelii R. Br. ex Oliv.

Neumann 923 (FR)

3.35/5.48

61.2***

5.58/13.28

41.24***

Yes

Quercus faginea Lam.

Benavides SP13_QUFA46

3.53/4.67

75.4***

4.67/8.06

58.0***

Yes

Rhabdophyllum calophyllum (De Wild. and T. Durand) Tiegh.

Hijmans 424 (L)

2.95/5.36

55.1***

5.36/7.62

70.3***

Yes

Acer pseudoplatanus L.

Benavides RO025

3.96/4.38

90.5

na

na

na

Afzelia africana Sm.

Küppers 1951 (FR)

2.86/3.30

86.9*

na

na

na

Bauhinia rufescens Lam.

Kahlheber 262 (FR)

4.26/5.68

75.0***

na

na

na

Betula pendula Roth

Benavides FIN03

3.54/4.45

79.5**

na

na

na

Brackenridgea zanguebarica Oliv.

Schultka 147 (FR)

3.11/3.65

85.2

na

na

na

Bridelia ferruginea Benth.

Hahn 978 (FR)

4.72/7.41

63.7***

na

na

na

Cassia sieberiana DC.

Kahlheber 925 (FR)

1.65/2.38

69.2***

na

na

na

Cassia singuena Delile

Hahn-Hadjali 1206 (FR)

2.92/3.91

74.8***

na

na

na

Cristaria integerrima Phil.

Schneider 3027 (FR)

4.61/7.37

62.5***

na

na

na

Crossopteryx febrifuga (Afzel. ex G. Don) Benth.

Küppers 796 (FR)

4.48/5.36

83.7***

na

na

na

Diospyros mespiliformis Hochst. ex A. DC.

Küppers 2131 (FR)

3.49/4.41

79.2***

na

na

na

Godoya antioquiensis Planch.

Uribe 2700 (U)

8.51/10.72d

79.4***

na

na

na

Landolphia heudelotii A. DC.

Neumann 738 (FR)

4.35/4.55

95.5

na

na

na

Lophira alata Banks ex C.F. Gaertn.

Leonard 1089 (FR)

1.98/2.71

73.3*

na

na

na

Medusagyne oppositifolia Baker

Friedmann 4444b (FR)

5.09/5.54d

91.8**

na

na

na

Ocotea calophylla Mez

Schneider 1238 (FR)

5.16/5.61

92.0**

na

na

na

Ouratea scottii Sastre

Jansen-Jacobs 1895 (U)

4.21/6.25

67.3***

na

na

na

Ouratea vaccinioides Engl.

Seele 736 (LZ)

2.29/3.17

72.4***

na

na

na

Saba senegalensis (A. DC.) Pichon

Schmidt 892 (FR)

3.03/3.57

85.1*

na

na

na

  1. *Differences significant at p < 0.05/2, ** 0.01/2 or *** 0.001/2 level (after Bonferroni correction)
  2. aThe structure of the very dense, parallel last order veins was not high enough in 2D X-ray images for vein density measurement
  3. bSome last order veins with low contrast
  4. cHighest order veins not distinct
  5. dThe 7 µm images provide full resolution of the leaf venation (as identified by comparison with cleared leaf images)