Fig. 4

Mutations in conserved splice sites of LHCB3 pre-mRNA substrate modulated the production of the spliced product. a The diagram shows sequence substitutions of conserved 5′ GU and 3′ AG splice sites (ss) of LHCB3 pre-mRNA substrate. DNA templates with different splice site mutations (M1 and M2) were synthesized (Integrated DNA Technologies, Inc., Coralville, IA, USA) for in vitro [³²P]-labelled RNA synthesis. b In vitro splicing of [³²P]-labeled wild type and two mutants (M1 and M2) of LHCB3 pre-mRNA substrate was carried as described before. Reactions were stopped after 3 h. [³²P]-RNA was isolated and analyzed by electrophoresis as above. RNA markers (M and M*) and schematic diagrams on the right were described in Fig. 2 legend