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Fig. 2 | Plant Methods

Fig. 2

From: An efficient transient expression system for gene function analysis in rose

Fig. 2

The effect of different infiltration buffers on transient expression efficiency in roses. 20-d old shoots were infiltrated with Agrobacterium carrying pKSN:LUC suspended with MS medium, infiltration buffer (IB) (2 mM Na3PO4, 50 mM MES, 0.5% glucose), and sterilized water with/without 100 μM acetosyringone. a Bright-field, b dark-field, c intensity of LUC bioluminescence quantified using Andor Solis image analysis software. Data are mean ± SEM of five biological replicates each with three technical repeats, 20 shoots were used in each technical repeat

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