Fig. 5
From: Segmentation of 3D images of plant tissues at multiple scales using the level set method
Cellular LSM used for the segmentation of nuclei. a Two-coloured confocal image of an Arabidopsis flower, were cell membrane and nuclei are taken on two distinct channels. b Segmentation of nuclei using level set method (erosion for initialisation \(d=1\), other parameters \(\alpha =0\), \(\beta =0\), \(\gamma =0.8\), \(s=1\), type = ‘g’). c Zoom on the segmented nuclei. d Distribution of the volume of nuclei and cells in the tissue. e Nuclear volume versus cell volume, a correlation coefficient of 0.58 was measured