Fig. 1
From: Segmentation of 3D images of plant tissues at multiple scales using the level set method
Description of the experimental context. a A side view of the aerial parts of an Arabidopsis thaliana plant. The boxed region indicates the position of the Inflorescence Meristem (IM) at the apical tip of the plant. b A top view of the IM, with mature flowers as well as young floral meristems visible. c A scanning electron micrograph of the IM with most flowers dissected away. Remaining flower buds are false-colored in violet. d A brightfield image of the same structure with a stage 3 flower bud indicated (arrow). e The dissected stem is stabilized on growth medium in a box suited to the water-dipping objective on the confocal microscope. f A 2D surface projection of the 3D image stack acquired on the confocal. g Individual slices from various positions along the Z axis indicate the 3D nature of the image stack. Z positions are provided. Scale bars indicate 25 μm