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Table 3 Agrobacterium-mediated transformation of switchgrass Alamo calluses without pre-selection of calluses types

From: Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum)

Vector

Experiment

No. of calluses inoculated

No. of hygromycin resistant callus after 6 weeks selection

No. of regenerated callus

No. of rooting seedling

No. of plants positive for genomic DNA PCR

Selection frequency (%)

pCAMBIA-EV

1

26

19

4

4

4

 

2

31

22

4

4

4

 

3

33

19

5

4

4

 

Total

90

60

13

12

12

100.0

pCAMBIA-RFP

1

26

18

4

5

2

 

2

34

23

6

5

5

 

3

35

22

7

4

4

 

Total

95

63

17

14

11

78.6

pCAMBIA1305.2

1

20

19

5

4

1

 

2

30

23

5

4

3

 

Total

50

42

10

8

4

50.0

  1. Selection efficiency: number of genomic DNA PCR positive switchgrass transformants divided by the regenerated and rooted hygromycin B resistant switchgrass seedlings