Fig. 1From: Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum)Callus induction of wild type switchgrass seeds. a Sterilized seeds on callus induction medium (CIM) at day zero. The embryo is indicated by the dashed oval. b Sterilized seeds on callus induction medium (CIM) at week one. The callus formation is initiated at the scutellum of the embryo (arrow), a tissue between the endosperm and coleoptile/coleorhiza. c Sterilized seeds on callus induction medium (CIM) at week two. d Sterilized seeds on CIM at week four. e Sterilized seeds on CIM at week sixBack to article page