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Fig. 3 | Plant Methods

Fig. 3

From: Robust genetic transformation of sorghum (Sorghum bicolor L.) using differentiating embryogenic callus induced from immature embryos

Fig. 3

Molecular confirmation of stable T0 transformants. a PCR detection of nptII transgene in randomly selected lines. Lanes from left to right: M = 1 kb plus DNA ladder, 2–18 transgenic lines, P plasmid DNA, Wt WT Tx430 DNA, NC water control, b copy number of nptII gene in 30 independent T0 transgenic plants analysed by digital droplet PCR (Error bar represents standard error in reading the copy number)

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Plant Methods

ISSN: 1746-4811