High-throughput detection of antioxidants in mulberry fruit using correlations between high-resolution mass and activity profiles of chromatographic fractions

Table 2 Identification of standard anti-oxidant compounds in the EtOAc extract of mulberry fruit using UHR 15-T FT-ICR mass spectrometry

#	Standard name	Formula	Calc. [M-H]⁻ [m/z]	Observed [M-H]⁻ [m/z]	Error [mDa]	S/N
1	Gentisic acid	C₇H₆O₄	153.01933	153.01933	0.00	24
2	Gallic acid	C₇H₆O₅	169.01425	169.01426	0.01	10.1
3	Caffeic acid	C₉H₈O₄	179.03498	179.03498	0.00	18.5
4	Luteolin	C₁₅H₁₀O₆	285.04046	285.04047	− 0.01	372.3
5	Kaempferol	C₁₅H₁₀O₆	285.04046	285.04047	− 0.01	372.3
6	Quercetin	C₁₅H₁₀O₇	301.03538	301.03538	0.00	710.1
7	Dihydroquercetin	C₁₅H₁₂O₇	303.05103	303.05104	− 0.01	370.6
8	Chlorogenic acid	C₁₆H₁₈O₉	353.08781	353.08780	0.01	191.4
9	Quercitrin	C₂₁H₂₀O₁₁	447.09329	447.09326	0.03	53.9
10	Quercetin-3-o-glucoside	C₂₁H₂₀O₁₂	463.08820	463.08820	0.00	467.9
11	Rutin	C₂₇H₃₀O₁₆	609.14611	609.14610	0.01	245.5

A UHR mass profile of the compounds in EAEM was obtained and all 11 SAOx were detected at high mass accuracy with measurement error < 0.05 mDa

ISSN: 1746-4811