Fig. 10

Active compound enrichment curves by different scoring methods. a The antioxidative compound enrichment curve (EI = 0.83) of the scoring method 1 was obtained for correlation coefficients C _j between normalized activity and mass profiles as previous SCAMP methods. b The antioxidative compound enrichment curve (EI = 0.98) of the scoring method 2. To compensate for the effects of concentration on antioxidative components included in the extract, we summed antioxidant mass peak intensities through all fractions (k) and multiplied this by the original C _j , resulting in the second improved scoring method. c The enrichment curve (EI = 0.99) for the third scoring method. If some components have the same mass but different structures, then those two components will present two separate chromatographic distributions and within different fractions. The grouping strategy was adopted to improve the performance of SCAMP by avoiding overestimation of the correlation coefficient by providing additional scores for multiple components within the same mass peak