Fig. 7
From: Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues
Western blot analysis to determine COX2 protein abundances in tobacco organs. Mitochondria isolated from young leaves, fully expanded leaves, roots, flowers and floral buds for western blot analysis using anti-COX2 antibody (provided by Prof. Thomas D. Fox, Cornell University, USA). For quantitative assessment of protein abundance in these tissues, dilution series of extracted mitochondrial proteins were loaded onto the gel for each tissue. Data were confirmed by analysis of three biological and technical replicates. Equal loading was further confirmed by silver staining of the gel