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Fig. 7 | Plant Methods

Fig. 7

From: Contrast-enhanced 3D micro-CT of plant tissues using different impregnation techniques

Fig. 7

Split section greyscale images of tomato leaflet with no contrast enhancement (a), 5 h (b) and 10 h (c) of contrast incubation by transpirational pull. In the unenhanced greyscale (a), leaf tissue is denoted by dark grey pixels, while air and crystalline deposits are marked by black and white pixels respectively. After 5 h of contrast incubation (b) faint traces of the contrast solution (light grey lines, see red arrows) are visible in the vasculature of the leaf (marked by grey pixels). After 10 h of incubation (c), the contrast solution invaded the plant tissue and oversaturation of the sample occurs. Leaf tissue is now replaced by light grey pixels, and no improvements to intercellular boundaries was observed over the conventional scans. All scale bars are set at 500 µm

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