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Fig. 5 | Plant Methods

Fig. 5

From: Contrast-enhanced 3D micro-CT of plant tissues using different impregnation techniques

Fig. 5

Non-registered comparison between conventional and contrast enhanced scans of tomato (cv. Bonaparte) mesocarp (left and right columns respectively). Greyscale image of the conventional scan a contains air “a” labelled by black pixels, while cells “c” are labelled by dark grey pixels. Greyscale images of the contrast enhanced scan b contains air “a” labelled by black pixels, cells “c” labelled by dark grey pixels as well as contrast fluid “ci” labelled by near white pixels in intercellular airspaces and boundaries. c, d The segmented cells in white. e, f Depicts the segmented cell volumes in 3D with arbitrary colors assigned, where separate cells in the same neighborhood are marked with a different color. g, h Removes debris and incomplete cells that intersected the VOI boundaries in respective control and contrast enhanced datasets. All scale bars are set at 500 µm while all bounding edges are 2000 µm

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