Fig. 4

Non-registered comparison between conventional and contrast enhanced scans of pear (cv. Conference) hypanthium (left and central columns respectively). Greyscale image of the conventional scan a contain air spaces in black pixels, as well as cells and brachysclereids in grey pixels labelled by “a”, “c” and “b” respectively in the image. Greyscale image of contrast enhanced scan b contain black pixels marking air spaces “a”, dark grey pixels marking cells “c”, light grey pixels in clusters marking brachysclereids “b”, near white pixels marking contrast flooded air spaces “ci”, as well as light grey pixels in lines marking intercellular boundaries. c, d Depict the segmented cells in white. e, f The segmented cell volumes in 3D with arbitrary colors, where individual cells in the same neighborhood are marked with a different color. g, h Removes incomplete cells that intersected the VOI boundaries in the respective control and contrast enhanced datasets. i The approximate size and localization of the brachysclereids along with a vascular bundle (elongated green structure) in the VOI. All scale bars present are 500 µm while all bounding edges are 2400 µm