Fig. 3

Registered comparison between conventional and contrast enhanced scans of apple (cv. Kanzi) hypanthium (left and central columns respectively). Greyscale images of the conventional scan a contains air spaces “a” in black pixels, as well cells “c” and vasculature “v” in grey pixels. Contrast enhanced greyscale image b is composed of air space “a”, cells “c”, vasculature “v” as well as contrast solution “ci”, which is represented by black, grey, light grey and white pixels respectively. Note that the contrast solution occupies not only intercellular spaces (marked by light grey lines), but a majority of the intercellular spaces were also invaded by the solution (such as the airspace marked by “ci”). c, d Depicts segmented cells in white. e, f Depict the segmented cell volumes in 3D with arbitrary colors, where individual cells in the same neighborhood are marked with a different color. g, h Remove debris and incomplete cells that intersected the VOI boundaries in the respective control and contrast enhanced datasets. i Demonstrates the 3D localization of the vasculature bundle found within the scanned sample. All scale bars present are 500 µm while all bounding edges are 2400 µm