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Table 2 Comparison of the TENS-CO method with standard DNA isolation procedures for the crop species used in the current study

From: Reproducible genomic DNA preparation from diverse crop species for molecular genetic applications

Method

DNA yield (µg/mg tissue)

DNA purity

A260:A230

A260:A280

Grass

Sugarcane (Saccharum spp. hybrids)

 TENS-CO

0.46 ± 0.05

2.04 ± 0.11

1.85 ± 0.01

 Standard SDS (modified from Tai and Tanksley [20])

0.10 ± 0.01

1.61 ± 0.07

1.96 ± 0.02

Cereal

Rice (Oryza sativa L. sp. japonica)

 TENS-CO

0.35 ± 0.05

2.18 ± 0.07

1.93 ± 0.01

 MO BIO PowerPlant Pro Kit

0.10 ± 0.01

2.02 ± 0.05

1.84 ± 0.01

Citrus

Sweet orange (Citrus sinensis L. cv. Hamlin)

 TENS-CO

0.64 ± 0.08

2.08 ± 0.04

2.00 ± 0.01

 Standard CTAB (modified from Chee et al. [43])

0.12 ± 0.01

2.32 ± 0.09

1.92 ± 0.02

Vegetables

Potato (Solanum tuberosum L. cv. Atlantic)

 TENS-CO

0.50 ± 0.04

2.23 ± 0.006

1.91 ± 0.04

 MO BIO PowerPlant Pro Kit

0.05 ± 0.004

2.41 ± 0.05

1.94 ± 0.03

 Synergy Plant DNA Kit

0.20 ± 0.01

2.18 ± 0.05

1.93 ± 0.02

Tomato (Solanum lycopersicum L. cv. Lance)

 TENS-CO

0.66 ± 0.06

2.10 ± 0.05

1.93 ± 0.01

 MO BIO PowerPlant Pro Kit

0.05 ± 0.004

1.87 ± 0.08

1.80 ± 0.01

 Synergy Plant DNA Kit

0.11 ± 0.01

1.93 ± 0.03

1.81 ± 0.01

  1. CTAB cetyltrimethylammonium bromide and SDS sodium dodecyl sulfate
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Plant Methods

ISSN: 1746-4811