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Table 1 Major steps of the TENS-CO protocol used for plant genomic DNA isolation

From: Reproducible genomic DNA preparation from diverse crop species for molecular genetic applications

(1) Extraction

TENS buffera and COb

5 M potassium acetate

(2) Precipitation

3 M sodium acetate (pH 5.2) (0.1× volume)

Ethanol (2.0× volume)

  1. a TENS buffer: 100 mM Tris (hydroxymethyl) aminomethane (Tris Base) (pH 8.0), 50 mM ethylenediamine-tetraacetic acid (EDTA) (pH 8.0), 500 mM sodium chloride (NaCl), 1% (w/v) sodium dodecyl sulfate (SDS), and 2% (v/v) 2-mercaptoethanol
  2. b CO chloroform:octanol (24:1)