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Fig. 3 | Plant Methods

Fig. 3

From: A cautionary note on the use of chromosome conformation capture in plants

Fig. 3

Chromosome conformation capture at the SOC1 locus of Arabidopsis using the NlaIII restriction enzyme. a Schematic diagram of SOC1 locus showing the positions of all NlaIII restriction sites (red arrowheads), and the fragments tested for interaction with a region spanning the Transcriptional Start Site (TSS; Fragment I). The pink blocks indicate the 5′ and 3′UTR and the orange blocks highlight the position of exons along the SOC1 locus. The graph represents the results of the 3C analysis on 3-week old wild-type (Col-0) seedlings using fragment I as 3C bait. Mean (± SD) for two biological replicates plotted. b A schematic representation of the top two interacting fragments (in brown) identified at the SOC1 locus with FspBI and NlaIII

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