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Fig. 2 | Plant Methods

Fig. 2

From: A cautionary note on the use of chromosome conformation capture in plants

Fig. 2

Reciprocal chromosome conformation capture (r3C) at the SOC1 locus of Arabidopsis using the FspBI restriction enzyme. a Schematic diagram of SOC1 locus showing the position of all FspBI restriction sites, and the fragments tested for interaction. The pink blocks indicate the 5′ and 3′UTR and the orange blocks highlight the position of exons along the SOC1 locus. The graph shows the outcome of the r3C analysis of SOC1 with FspBI using fragment X as bait. The experiments were performed on the same 3C libraries used in the original 3C experiment presented in Fig. 1. Mean (± SD) derived from three biological replicates is plotted. b A heat map summarizing and comparing the 3C and r3C interaction profile obtained with FspBI. The cross indicates the fragment used as bait. Note that the highest reciprocal contact frequency was identified between fragment VII and X. The intensity of blue colouring is an indication of the relatively interaction frequency (scale from 0-1.0)

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