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Fig. 6 | Plant Methods

Fig. 6

From: Rapid in situ 13C tracing of sucrose utilization in Arabidopsis sink and source leaves

Fig. 6

Differential carbon utilization of 13C sucrose by sink and source leaf metabolism. Relative 13C enrichments of metabolite pools from sink leaves (a) or source leaves (b) normalized to 13C sucrose enrichment per sample (Mean ± standard error, n ≥ 8 mean values per plant of leaves from position P1–P3). Dark to light blue colour coding of relative 13C enrichment indicates progressing 13C dilution in the analysed metabolite pools (n.a., data not available). Grey trapezoids (isosceles) represent the decrease in 13C enrichment between two neighbouring metabolites of the pathway scheme. The length of the trapezoid base is proportional to the 13C enrichment of the corresponding metabolite. c Bi-plot of relative 13C enrichments in sink compared to source leaves. d Bi-plot of the coefficients of variation of relative 13C enrichment measurements in sink compared to source leaves. e Differential matrix of relative 13C enrichment in metabolite pools of sink compared to source leaves. For this purpose two distance matrices of 13C enrichment ratios among all monitored metabolites were calculated separately from sink and source leaf data (Additional file 9: Table S5). The log10-transformed source leaf matrix was subtracted from the log10-transformed sink leaf matrix (blue negative, red positive) to obtain the final differential matrix. Significance of the log10-transformed 13C enrichment ratios within the sink and source leaf matrices was tested by Student’s t test (P ≤ 0.001, boxes indicate differential ratios that were significant either only in sink or only in source leaves). Hierarchical clustering using Euclidian distance and complete linkage sorted metabolites according to their differential enrichment ratios into five clusters with distance cut-off 1.5. Glutamine was determined as sum of glutamine and pyroglutamate

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