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Fig. 5 | Plant Methods

Fig. 5

From: Rapid in situ 13C tracing of sucrose utilization in Arabidopsis sink and source leaves

Fig. 5

Comparison of the relative changes of metabolites in sink compared to source leaves based on 13C labeled (a) or total metabolite pool sizes of the same samples (b). Complete A. thaliana rosettes were labeled 4 h by 100 mM 13C sucrose using the HFA. All metabolite pool sizes were normalized to internal standard and sample dry weight [38]. Leaf positions P2–P3 were grouped to represent sink leaves, leaf positions P6–P7 were identified as source leaves. Significance of the sink to source leaf ratios was tested by Student’s T test (P ≤ 0.01; black boxes). Ratios are means (± standard error), n = 6–10. A metabolite that was labeled only in sink leaves is indicated by (+). Glutamine was determined as sum of glutamine and pyroglutamate

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