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Fig. 4 | Plant Methods

Fig. 4

From: Rapid in situ 13C tracing of sucrose utilization in Arabidopsis sink and source leaves

Fig. 4

Distribution and reproducibility analysis of labeled sucrose in A. thaliana Col-0 rosette applied by the hypocotyl feeding assay. a Average 13C enrichment of sucrose in rosettes across all leaves (P1–P6). 13C enrichment values of single leaves of each rosette were averaged after hypocotyl feeding with a 20 mM and a 100 mM 13C-sucrose solution. The range of 13C enrichment was 7–26% and 12–68%, respectively. Error bars represent standard deviation (n = 6). b Average 13C enrichment of sucrose in leave positions across eight rosettes. 13C enrichment values of leaf position were averaged after hypocotyl feeding with a 20 mM and a 100 mM 13C-sucrose solution. The range of 13C enrichment was 7–22% and 36–50%, respectively. Error bars represent standard deviation (n = 8). c Average 13C pool size (arbitrary units) of sucrose calculated from the sum of labeled and non-labeled sucrose and 13C enrichment data. Error bars represent standard error (n = 6). d Improvement of averaged relative standard deviation of 13C enrichment (avg. RSD %) across 22 annotated and labeled metabolites except sucrose by data processing: (Step I) Relative standard deviation was calculated of each metabolite separately across P1–P3 or P5–P6 leaves and subsequently averaged without further data processing. (Step II) Relative standard deviation of 13C enrichment was calculated after signal to noise thresholding obtained by non-labeled control samples. (Step III) Average RSD was further reduced after internal normalization to the 13C enrichment value of sucrose in each sample. Error bars represent standard error (n = 22)

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