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Table 1 Candidate reference genes and primer sequences

From: Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress

Gene

Gene code

Primer sequences (forward/reverse)

Amplicon length (bp)

log2(drought/CK)

Tm (°C)

E (%)

R2

EF1α

PGSC0003DMG400023270

GATGGTCAGACCCGTGAACA

106

0.148

60.9

102.95

0.999

  

CCTTGGAGTACTTCGGGGTG

     

CUL3A

PGSC0003DMG400001321

AGCATCGGGTTGTTGTGGAT

170

0.173

59.0

95.56

0.998

  

TCCTGAATAGAGCTTCTCCCCA

     

GAPDH

PGSC0003DMG400015253

GCTCATTTGAAGGGTGGTGC

151

0.257

58.8

101.69

0.997

  

AGGGAGCAAGGCAATTTGTG

     

sec3

PGSC0003DMG402015451

GCTTGCACACGCCATATCAAT

160

0.084

58.0

100.88

0.995

  

TGGATTTTACCACCTTCCGCA

     

tubulin

PGSC0003DMG400009938

GGGAATAACTGGGCGAAAGGT

134

− 0.185

60.0

97.00

0.996

  

CCTCCACCAAGTGAGTGACAA

     

L8

PGSC0003DMG400025015

GTTGGTAATGTGTTGCCGCT

172

0.328

58.8

102.96

0.996

  

TGGCACCTGATGGGAGCTTA

     

APRT

PGSC0003DMG400021527

CGTATCGCTGGGATTGCTTC

177

0.065

58.9

98.31

0.995

  

TGCTTCAATACCTGCAACCAC

     

actin

PGSC0003DMG400023429

AGGAGCATCCTGTCCTCCTAA

180

− 0.315

60.0

103.40

0.998

  

CACCATCACCAGAGTCCAACA

     
  1. log 2 (drought/CK) the log2 value of the ratio of drought treatment to control reads per kilo bases per million reads, E PCR efficiency, Tm annealing temperature, R 2 regression coefficient