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Table 2 Comparison of conventional macroscale and improved microscale methods for extracted bound phenolic acids founded in whole maize hybrids kernels

From: An improved microscale method for extraction of phenolic acids from maize

  p-Coumaric acida (µg/g dw) trans-Ferulic acida (µg/g dw) 5-5 DFAb (µg FAE/g dw) DFPb (µg FAE/g dw)
Conv Improve Conv Improve Conv Improve Conv Improve
Minimum 6.0 34.4 131.8 673.8 8.6 22.5 6.6 13.4
Mean 19.4 74.2 249.7 1029.9 12.9 64.9 20.2 25.0
Maximum 30.6 138.6 427.5 1702.7 17.3 131.5 31.5 49.9
LSD 18.6 10.9 270.1 121.9 8.7 8.2 18.2 19.9
C.V. 58.9 8.9 53.8 7.2 40.5 7.6 54.7 48.1
ANOVA *** *** *** *
  1. Conv conventional method. Improve improved method. DFA diferulic acid, DFP diferuloylputrescine. Significant differences between the methods were established by ANOVA (α = 0.05), NS no significance, * p < 0.05; ** p < 0.001; *** p < 0.001. LSD least significant difference (α = 0.05)
  2. aResults are expressed as the average of three replicates as µg per g dry weight (µg/g dw)
  3. bResults are expressed as the average of three replicates as µg FAE/g dw: µg of ferulic acid equivalents per g dw