From: Improved method for genomic DNA extraction for Opuntia Mill. (Cactaceae)
Mondragón-Jacobo et al. [11] | Griffith and Porter [13] | This protocol |
---|---|---|
They tried to use young tissues, avoiding older ones because their higher content of fiber and cuticular wax | They tried to use epidermal tissue free of waxes | We can use tissue from any part of the plant |
They used β-mercaptoethanol | They used β-mercaptoethanol | We did not use β-mercaptoethanol |
8000 mg of cactus pear tissue | 30–50 mg of epidermal tissue | 2–3 mg of tissue from every part of the plant |
They used more CTAB (25 ml) | They used more CTAB (15 ml) | We used few CTAB (0.7 ml) |
They used more chloroform-isoamyl alcohol (10 ml) | They used more chloroform-isoamyl alcohol (5 ml) | We used few chloroform-isoamyl alcohol (0.75 ml) |
They used ethanol (8.7 ml) | They used more isopropanol (5 ml) | We used few isopropanol (0.4 ml) |
They used bigger and expensive tubes (15 ml) | They used bigger and expensive tubes (15 ml) | We used smaller tubes (2 ml) |
They used RNAse to eliminate RNA | They did not use RNAse | We did not use RNAse |