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Table 4 Comparison among three different protocols to obtain total genomic DNA using NanoDrop

From: Improved method for genomic DNA extraction for Opuntia Mill. (Cactaceae)

Species Mondragón-Jacobo et al. [11] Griffith and Porter [13] This protocol
DNA yield (ng/µl) OD ratio 260.280 DNA yield (ng/µl) OD ratio 260.280 DNA yield (ng/µl) OD ratio 260.280
1. Opuntia auberi Pfeiff. 256 1.4 423 1.7 1600 1.9
2. Opuntia decumbens Salm-Dyck 35 1.7 30 1.9 2930 1.9
3. Opuntia delafuentiana Martínez-González et al. 75 1.6 56 1.9 4937 1.8
4. Opuntia depressa Britton and Tose 95 1.9 73 1.8 8755 1.9
5. Opuntia durangensis Britton and Tose 134 1.5 123 1.7 5835 1.9
6. Opuntia ficus-indica Mill. 34 1.8 258 1.8 3829 2.0
7. Opuntia heliabravoana Scheinvar 198 1.6 43 1.8 8743 1.8
8. Opuntia huajuapensis Bravo 57 1.5 78 1.7 1573 1.9
9. Opuntia joconostle F.A.C. Weber 86 1.9 196 1.0 8375 1.8
10. Opuntia lasiacantha Pfeiff. 67 1.7 356 1.7 2943 1.9
11. Opuntia leiascheinvariana Martínez-González 110 1.8 98 1.9 3980 1.9
12. Opuntia leucotricha DC. 248 1.5 34 1.8 3789 1.9
13. Opuntia matudae Scheinvar 93 1.7 63 1.8 7947 1.9
14. Opuntia megacantha Salm-Dyck 117 1.6 78 1.8 7000 1.8
15. Opuntia microdasys Pfeiff. 44 1.5 39 1.7 6578 1.9
16. Opuntia oligacantha Förster 87 1.8 70 1.9 2395 1.8
17. Opuntia olmeca Joel Pérez et al. 94 1.5 57 1.7 9200 1.9