Table 2 Primers used in the amplification and sequencing of the DNA fragments
From: Improved method for genomic DNA extraction for Opuntia Mill. (Cactaceae)
Locus/segment | Name | Sequence 5′–3′ | Tm (°C) |
|---|---|---|---|
ITS | ITS5 | GGAAGTAAAAGTCGTAACAAGG | 57 |
ITS4 | TCCTCCGCTTATTGATATGC | 57 | |
rbcL | 1f | ATGTCACCACAAACAGAAAC | 56 |
724r | TCGCATGTACCTGCAGTAGC | 56 | |
cox3 | Cox3f | CCGTAGGAGGTGTGATGT | 51 |
Cox3r | CTCCCCACCAATAGATAGAG | 51 |