Table 1 Genomic DNA (gDNA) concentration and quality extracted from 17 tissue samples of Opuntia Mill. using the improved extraction method
From: Improved method for genomic DNA extraction for Opuntia Mill. (Cactaceae)
Species | PicoGreen ng/µl | NanoDrop ng/µl | C B ratio PicoGreen concentration/Nanodrop concentration | A260/A280 NanoDrop | A260/A230 NanoDrop |
|---|---|---|---|---|---|
1. Opuntia auberi Pfeiff. | 1250 | 1500 | 0.83 | 1.9 | 2.1 |
2. Opuntia decumbens Salm-Dyck | 3199 | 3642 | 0.87 | 1.9 | 2.2 |
3. Opuntia delafuentiana Martínez-González et al. | 8021 | 8126 | 0.98 | 1.9 | 2.2 |
4. Opuntia depressa Britton and Rose | 2191 | 2588 | 0.84 | 1.9 | 2.0 |
5. Opuntia durangensis Britton and Rose | 8220 | 8853 | 0.92 | 1.8 | 2.1 |
6. Opuntia ficus-indica Mill. | 5898 | 6196 | 0.95 | 1.9 | 2.1 |
7. Opuntia heliabravoana Scheinvar | 8341 | 9147 | 0.91 | 2.0 | 1.9 |
8. Opuntia huajuapensis Bravo | 3624 | 4497 | 0.80 | 1.9 | 2.1 |
9. Opuntia joconostle F. A. C. Weber | 1091 | 1304 | 0.83 | 1.8 | 2.2 |
10. Opuntia lasiacantha Pfeiff. | 1892 | 2088 | 0.90 | 1.9 | 2.1 |
11. Opuntia leiascheinvariana Martínez-González | 4799 | 5407 | 0.88 | 1.9 | 2.2 |
12. Opuntia leucotricha DC. | 6258 | 7000 | 0.89 | 1.9 | 2.2 |
13. Opuntia matudae Scheinvar | 2354 | 2802 | 0.84 | 1.9 | 2.2 |
14. Opuntia megacantha Salm-Dyck | 6895 | 7861 | 0.87 | 1.8 | 2.1 |
15. Opuntia microdasys Pfeiff. | 7526 | 8592 | 0.87 | 1.9 | 2.1 |
16. Opuntia oligacantha Förster | 1548 | 1897 | 0.81 | 1.8 | 2.2 |
17. Opuntia olmeca Joel Pérez et al. | 2112 | 2568 | 0.82 | 1.9 | 2.1 |