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Table 1 Comparison of five different DNA extraction methods for P. wickerhamii POL-1 strain in terms of purity and yield

From: An optimized method for high quality DNA extraction from microalga Prototheca wickerhamii for genome sequencing

Method

Absorbance

Concentration (ng/µL)

260:280

260:230

Liquid nitrogen (LN)

2.08 ± 0.02

2.23 ± 0.26

60.96 ± 0.16

CTAB (C)

1.84 ± 0.03

2.59 ± 0.02

12.07 ± 0.7

Enzyme cocktail (EC)

−7.97 ± 19.25

0.15 ± 0.02

1.6 ± 0.01

GeneMATRIX Bacterial & Yeast (K1)

1.32 ± 0.50

0.51 ± 0.07

2.28 ± 0

GeneMATRIX Plant & Fungi (K2)

0.70 ± 0.29

0.53 ± 0.17

3.85 ± 0.02

Newly designed protocol (N)

2.02 ± 0.03

1.97 ± 0.07

74.2 ± 0.56