Development of TaqMan probes targeting the four major celiac disease epitopes found in α-gliadin sequences of spelt (Triticum aestivum ssp. spelta) and bread wheat (Triticum aestivum ssp. aestivum)

Table 1 Genetic material used to analyze the α-gliadin canonical epitope expression with TaqMan probes

Species	Genome	Name	Accession name	Accession number	Germplasm	Provenance country
Triticum aestivum ssp. spelta	ABD	BEL08	69Z6,485	PI348315	USDA (Washington, USA)	Belgium
		DK01	DN-2267	PI361811	USDA (Washington, USA)	Denmark
		SPA03	69Z6,752	PI348572	USDA (Washington, USA)	Spain
		BUL04	Ungarn	PI295063	USDA (Washington, USA)	Bulgaria
		GER11	69Z6,275	PI348114	USDA (Washington, USA)	Germany
		GER12	69Z6,282	PI348120	USDA (Washington, USA)	Germany
		TAD06	–	K 52437	VIR (Saint Petersburg, Russia)	Tajikistan
		SWI23	69Z6,93	PI347939	USDA (Washington, USA)	Switzerland
		US06	69Z5,73	PI355595	USDA (Washington, USA)	USA
		Iran77d	Iran 77d	CGN 06533	CGN (Wageningen, The Netherlands)	Iran
		IRA03	Iran 416A	CGN12270	CGN (Wageningen, The Netherlands)	Iran
Triticum urartu	A	LB01	G3178	PI 428293	USDA (Washington, USA)	Lebanon
Aegilops speltoides	S*	TR08	2733	PI 170204	USDA (Washington, USA)	Turkey
Aegilops tauschii	D	TR10	79TK057-322-1	PI 486267	USDA (Washington, USA)	Turkey

The 11 spelt accessions are contrasted accessions previously selected after a genetic diversity analysis [14]. T. urartu, Ae. speltoides and Ae. tauschii are diploid species representative of the three ancestral genomes (A, B and D, respectively) of spelt and bread wheat. They were selected to validate the specificity of the designed TaqMan probes
USDA United States Department of Agriculture, VIR Vavilov Institute of Plant Genetic Resources, CGN Center for Genetic Resources
* The B genome is hypothesized to be an altered S genome; Ae. sepltoides is therefore taken as the closest representative of the B genome

ISSN: 1746-4811