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Fig. 4 | Plant Methods

Fig. 4

From: Development of TaqMan probes targeting the four major celiac disease epitopes found in α-gliadin sequences of spelt (Triticum aestivum ssp. spelta) and bread wheat (Triticum aestivum ssp. aestivum)

Fig. 4

Expression profile of the cumulated canonical epitopes and the global α-gliadins among spelt and diploid accessions. a Relative quantification of the expression of the four cumulated canonical α-gliadin epitopes affecting CD patients among the 11 contrasted spelt accessions and the three diploid species T. urartu, Ae. speltoides and Ae. tauschii. The relative quantity of each epitope in the α-gliadin transcripts was calculated by the \(2^{{\Delta {\text{C}}_{\text{t}} }}\) method through the normalization of qPCR values to those obtained for the four most stable reference genes and the calibration to a unique α-gliadin clone displaying the four canonical epitopes in one copy. b Relative quantification of the expressed α-gliadin sequences in the same accessions. The sample with the lowest Ct value was chosen as calibrator. Error bars represent the standard error of the mean and the letters displayed above denote significant differences highlighted by Tukey’s multiple comparison test

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