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Fig. 3 | Plant Methods

Fig. 3

From: Development of TaqMan probes targeting the four major celiac disease epitopes found in α-gliadin sequences of spelt (Triticum aestivum ssp. spelta) and bread wheat (Triticum aestivum ssp. aestivum)

Fig. 3

Relative abundance of the four canonical α-gliadin epitopes among contrasted spelt accessions and diploid species. Developed TaqMan probes were used for the relative quantification of the expression of the four major α-gliadin T-cell stimulatory epitopes in their canonical form among 11 contrasted spelt accessions and the three diploid accessions representative of the ancestral genomes of spelt and bread wheat: T. urartu (LB01, A genome), Ae. speltoides (TR08, B genome) and Ae. tauschii (TR10, D genome). The relative quantities were calculated by dividing the \(2^{{\Delta {\text{C}}_{\text{t}} }}\) values by a normalization factor obtained through the expression analysis of four stable reference genes. Data are presented with standard error of the mean and significant differences detected by Tukey’s multiple comparison test are shown by different letters

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Plant Methods

ISSN: 1746-4811