Fig. 2From: Development of TaqMan probes targeting the four major celiac disease epitopes found in α-gliadin sequences of spelt (Triticum aestivum ssp. spelta) and bread wheat (Triticum aestivum ssp. aestivum)geNorm output charts of M and V values for the six tested reference genes. a Average expression stability M of the remaining reference genes after stepwise exclusion of the least stable gene. b Pairwise variation V between two sequential normalization factors containing an increased number of reference genes, calculated for the determination of the optimal number of reference genes for normalizationBack to article page