Microaspiration of Solanum tuberosum root cells at early stages of infection by Globodera pallida

Table 1 Primer sequences used for amplification of cDNA samples synthesized from RNA isolated from microaspirated Globodera pallida infected and un-infected potato roots cells

Gene	Primer sequence (5′–3′)	Product size (bp)	Genbank accession number
Elongation factor	Forward AACCGCTGAGAACTTCCGAG Reverse CGACCCAACAAGACACAAGC	566	AJ536671.1
Cinnamyl alcohol dehydrogenase	Forward TGCACATAACAGGGGAGCTG Reverse ATTACAGAGGCGCAACCAGG	579	JQ619511.1
6-Phosphogluconate dehydrogenase	Forward TTGGTGGGAGGTTTCTCGTG Reverse CCTGCCAATTTGGTTCAGGC	502	XM_006363489.2
NADH-quinone oxidoreductase subunit C	Forward CCAAGACTAAGGCCGAGCAA Reverse GGACGGATCACCTGTCGATC	520	AY056140.1
CHTB4 (Chitinase)	Forward CTGTTGCAGCAATTTCGGCT Reverse AAATTTGGATGGGGCCTCGT	576	U02608.1

Except for elongation factor, all primers were designed using gene sequences from RNAseq data. Therefore, the Genbank accession numbers given here are of identical sequences found in the NCBI

ISSN: 1746-4811