Fig. 5

Analysis of barley ovule traits by pistil clearing. a Ovule phenotypes were examined in nine cultivars of 2-row spring barley. Traits such as ovule area, nucellus area, antipodal number, antipodal area, nucellus proportion and integument width (see Fig. 4) were compared between the cultivars. Error bars show standard error. b Heat map showing the normalised trait values (between 0 and 1) for 11 ovule traits in the 9 examined cultivars. Cultivars and traits were clustered via hierarchical clustering. c Correlation analysis of 11 different ovule traits. The size and colour of the circles indicates the degree of trait correlation, which is also indicated via a numerical value. R-squared values greater than 0.95 are indicated via purple boxes. INT_W = integument width, NUC_% = nucellus proportion, ANT_N = antipodal number, OV_A = ovule area, NUC_A = nucellus area, OV_T = ovule transverse width, ES_L = embryo sac longitudinal height, OV_L = ovule longitudinal height, ES_T = embryo sac transverse width, ANT_A = entire antipodal area, ES_A = embryo sac area