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Fig. 3 | Plant Methods

Fig. 3

From: An optimised clearing protocol for the quantitative assessment of sub-epidermal ovule tissues within whole cereal pistils

Fig. 3

Structural details of the ovule are visible in cleared barley pistils (H. vulgare cv. Host). Images presented as composites, generated by merging optical sections. a Mature barley ovule imaged at ×10 magnification without dissection from the pistil. bd Cellular resolution may be achieved with a ×40 objective, allowing clear visualisation of the egg cell nucleus, synergid nuclei, polar nuclei and antipodal cell nuclei using the “long method” i.e. incubation for at least 10 weeks (10w) in Hoyer’s solution (b). The integument layers are also visible (c). Similar cellular resolution may also be achieved in samples processed with a 10-day (10d) “short” method (d). acn = antipodal cell nuclei, ccv = central cell vacuole, ecn = egg cell nucleus, ii = inner integument, nuc = nucellus, oi = outer integument, pn = polar nuclei, pc = pericarp, scn = synergid cell nuclei. The embryo sac is indicated by a dashed white line

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