Table 1 Optimal sample concentrations (mg ml−1) for quantification of condensed tannins using improved acetone-based butanol-HCl assay
Samples | Species | Growing conditions | Tissue type | Soluble CT (sample/solvent) | Insoluble CT (pellet/reagent) | Total CT (sample/reagent) |
|---|---|---|---|---|---|---|
Fresh leaf_MYB115/353(4) | Populus tremula × tremuloides | Greenhouse grown, well fertilized | Green leaf | 5.0 | 1.3 | 0.6 |
Fresh leaf_WT353 | Populus tremula × tremuloides | Greenhouse grown, well fertilized | Green leaf | 10.0 | 7.8 | 3.9–9.7 |
Fresh leaf_Low N_WT 353 | Populus tremula × tremuloides | Greenhouse grown, nitrogen limited | Green leaf | 10.0 | 7.8 | 3.9–9.7 |
Roots (young)_Low N | Populus tremula × tremuloides | Greenhouse grown, nitrogen limited | Live root | 5.0 | 1.3 | 0.6 |
Roots (mid-sized)_Low N | Populus tremula × tremuloides | Greenhouse grown, nitrogen limited | Live root | 5.0 | 1.3 | 0.6 |
Roots (older)_Low N | Populus tremula × tremuloides | Greenhouse grown, nitrogen limited | Live root | 10.0 | 2.6 | 1.3 |
Litter_LTLN | Populus angustifolia | Field grown | Abscised leaf | 5–10 | 1.3–2.6 | 1.3 |
Litter_HTLN | Populus angustifolia | Field grown | Abscised leaf | 5.0 | 1.3 | 0.6 |
Litter_LTHN | Populus angustifolia | Field grown | Abscised leaf | 5–10 | 1.3–2.6 | 1.3 |
Litter_HTHN | Populus angustifolia | Field grown | Abscised leaf | 5.0 | 1.3 | 0.6 |
Litter_FdLN | Pseudotsuga menziesii | Field grown | Abscised leaf | 20.0 | 5.2 | 3.9 |
Litter_FdHN | Pseudotsuga menziesii | Field grown | Abscised leaf | 20.0 | 5.2 | 3.9 |
Decayed litter_Poplar | Populus angustifolia | Field grown and decayed | Decayed abscised leaf | Not detected | 19.5–39 | 11.7 |
Decayed litter_Douglas-fir | Pseudotsuga menziesii | Field grown and decayed | Decayed abscised leaf | 39 to not detected | 31–39 | 11.7 |