Table 1 Numbers of sites at which one or two nt are mismatched within a 21-bp sequence region in the alignments of individual gene fragment to be used for building the TRV-UBC12/10/28/31 VIGS construct and corresponding region of other eight group III members
E2 | NbUBC12 fragment | NbUBC10 fragment | NbUBC28 fragment | |||
|---|---|---|---|---|---|---|
1/21 | 2/21 | 1/21 | 2/21 | 1/21 | 2/21 | |
NbUBC8 | 1 | 0 | 0 | 2 | 0 | 1 |
NbUBC9 | 1 | 1 | 1 | 1 | 1 | 0 |
NbUBC10 | – | – | – | – | – | – |
NbUBC11 | 0 | 1 | 0 | 0 | 0 | 0 |
NbUBC12 | – | – | – | – | – | – |
NbUBC28 | – | – | – | – | – | – |
NbUBC29 | 0 | 0 | 0 | 1 | 0 | 0 |
NbUBC30 | 1 | 1 | 2 | 1 | 1 | 0 |
NbUBC31 | – | – | – | – | – | – |
NbUBC38 | 1 | 0 | 0 | 3 | 0 | 1 |
NbUBC39 | 0 | 1 | 0 | 0 | 0 | 1 |
NbUBC40 | 0 | 2 | 0 | 1 | 0 | 1 |