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Fig. 7 | Plant Methods

Fig. 7

From: Elucidating the role of highly homologous Nicotiana benthamiana ubiquitin E2 gene family members in plant immunity through an improved virus-induced gene silencing approach

Fig. 7

Sequence alignments of three re-selected gene fragments used for silencing NbUBC12, 10, 28 and 31 and corresponding fragments of other group III E2 genes. The DNA fragments selected by SGN tool for VIGS were further optimized to ensure at least 3 nt mismatches within any 21-nt DNA stretch. The DNA fragment from NbUBC12 was designed to silence NbUBC12; the DNA fragments from NbUBC10 and NbUBC28 were designed to silence NbUBC10, 28 and 31. The alignments of optimized DNA fragments from NbUBC12 (a), NbUBC10 (b), and NbUBC28 (c), respectively with the corresponding region from NbUBC8, 9, 11, 29, 30, 38, 39 and 40 were shown. Color black denotes high consensus nucleotide while red denotes low and neutral consensus nucleotide. The sequences underlined in blue were the 21-bp DNA stretches within which at least 3 nt are mismatched. Numbers at the right of the underlines denote the counts of mismatched nucleotides in 21-bp DNA stretches

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Plant Methods

ISSN: 1746-4811