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Fig. 5 | Plant Methods

Fig. 5

From: Elucidating the role of highly homologous Nicotiana benthamiana ubiquitin E2 gene family members in plant immunity through an improved virus-induced gene silencing approach

Fig. 5

Sequence alignments of the three gene fragments used for building the VIGS construct TRV-UBC12/10/28/31 and the corresponding region of other group III E2 members. The alignments of gene fragments from NbUBC12 (a), NbUBC10 (b), and NbUBC28 (c), respectively with the corresponding region from NbUBC8, 9, 11, 29, 30, 38, 39 and 40 were shown. The DNA fragments from NbUBC10 and NbUBC28 were designed to silence NbUBC10, 28 and 31. Color black denotes high consensus while red denotes low and neutral consensus. The upper Arabic numerals designate the positions of the nucleotides. The sequences underlined in blue were 21-bp DNA stretches within which one nt is mismatched. The sequences underlined in red were 21-bp DNA stretches within which two nt are mismatched. Numbers at the right of the underlines denote the counts of mismatched nucleotides in the 21-bp DNA stretches. The non-underlined regions of each alignment have more than 2 mismatched nt within any 21-bp DNA stretches

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