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Table 3 Activated charcoal supplementation of regeneration media

From: A rapid and efficient in vitro regeneration system for lettuce (Lactuca sativa L.)

Medium

Basal salt mix

Sugar

Cytokinins (mg/l)

Auxins (mg/l)

AC (mg/l)

Control

MS 1×

Sucrose 3%

BA 2

NAA 0.1

–

M15

MS 1×

Sucrose 3%

BA 2.5

NAA 0.125

20

M16

MS 1×

Sucrose 3%

BA 5

NAA 0.25

20

M17

MS 1×

Sucrose 3%

BA 2.5

NAA 0.125

200

M18

MS 1×

Sucrose 3%

BA 5

NAA 0.25

200

M19

MS 1×

Sucrose 3%

BA 10

NAA 0.5

200

M20

MS 1×

Sucrose 3%

BA 5

NAA 0.25

2000

M21

MS 1×

Sucrose 3%

BA 10

NAA 0.5

2000

  1. All media pH was adjusted to 5.7, solidified with 7 g/l agar and autoclaved at 121 °C and 103 kPa for 20 min
  2. AC activated charcoal, MS Murashige and Skoog modified basal salts with Gamborg vitamins, BA benzylaminopurine, NAA naphthaleneacetic acid
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Plant Methods

ISSN: 1746-4811