A rapid and efficient in vitro regeneration system for lettuce (Lactuca sativa L.)

Table 2 Nutritional and hormonal variations of regeneration media

Medium	Basal salt mix	Sugar	Cytokinins (mg/l)	Auxins (mg/l)
Control	MS 1×	Sucrose 3%	BA 2	NAA 0.1
M1	SH 1×	Sucrose 3%	BA 2	NAA 0.1
M2	MS 1×	Glucose 1.5%	BA 2	NAA 0.1
M3	MS 1×	Sucrose 3%	BA 0.09	NAA 0.1
M4	MS 1×	Sucrose 3%	KIN 2	NAA 0.1
M5	MS 1×	Sucrose 3%	TDZ 2	NAA 0.1
M6	MS 1×	Sucrose 3%	ZEA 2	NAA 0.1
M7	MS 1×	Sucrose 3%	BA 1	NAA 0.05
M8	MS 1×	Sucrose 3%	BA 3	NAA 0.15
M9	MS 1×	Sucrose 3%	BA 4	NAA 0.2
M10	MS 1×	Sucrose 3%	BA 5	NAA 0.25
M11	MS 1×	Sucrose 3%	BA 6	NAA 0.3
M12	MS 1×	Sucrose 3%	BA 7	NAA 0.35
M13	MS 1×	Sucrose 3%	BA 8	NAA 0.4
M14	MS 1×	Sucrose 3%	BA 9	NAA 0.45

All media pH was adjusted to 5.7, solidified with 7 g/l agar and autoclaved at 121 °C and 103 kPa for 20 min
MS Murashige and Skoog modified basal salts with Gamborg vitamins, SH Schenk and Hildebrandt modified basal salts with vitamins, BA benzylaminopurine, KIN kinetin, TDZ thidiazuron, ZEA zeatin, NAA naphthaleneacetic acid

ISSN: 1746-4811