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Table 1 Tissue culture media originally used on ‘RSL NFR’

From: A rapid and efficient in vitro regeneration system for lettuce (Lactuca sativa L.)

Medium

Basal salt mix

Sugar

Cytokinins (mg/l)

Auxins (mg/l)

Germination

MS 1/2×

Sucrose 1%

–

–

Shoot induction

MS 1×

Sucrose 3%

BA 2

NAA 0.1

Root induction

MS 1×

Sucrose 3%

–

IBA 1

  1. All media pH was adjusted to 5.7, solidified with 7 g/l agar and autoclaved at 121 °C and 103 kPa for 20 min. Shoot induction medium was used as a control for all optimization experiments
  2. MS Murashige and Skoog modified basal salts with Gamborg vitamins, BA 6-benzylaminopurine, NAA naphthaleneacetic acid, IBA indole-3-butyric acid
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Plant Methods

ISSN: 1746-4811