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Fig. 5 | Plant Methods

Fig. 5

From: A comprehensive technique for artificial hybridization in Chickpea (Cicer arietinum)

Fig. 5

Parental preparation for crossing. Emasculation by keel petal incision method: a hooded flower bud chosen for emasculation of female flower. b Sepal removal for the keel petal incision. Red line shows the site of incision. c Flower bud showing the anther after keel petal incision. d Emasculated flower bud showing the stigma. Emasculation by petal removal method: e female flower bud selected for anther removal by petal excision method. f Detached sepals from bud. g Removal of standard, wing and keel petals exposing anther and stigma. h Emasculated female flower bud for cross-pollination. Pollen collection from male flower: i half open male flower for pollen collection. j Excision of sepals to expose the bud for petal removal. k Standard, wing and keel petal removal to collect the mature pollen. l Pollen collection on petriplate from 2 to 3 male flowers for cross-pollination. Bars 1 mm

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