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Fig. 8 | Plant Methods

Fig. 8

From: In vivo label-free mapping of the effect of a photosystem II inhibiting herbicide in plants using chlorophyll fluorescence lifetime

Fig. 8

TPE-FLIM images obtained from in vivo time course experiments in Triticum aestivum leaves. a Shows intensity merged fluorescence lifetime images of an untreated leaf at different time points. b Shows intensity merged lifetime images of a treated leaf at different time points showing an increase in lifetime compared to a. All the measurements were carried out using a Leica TCS SP5 system and analyzed using FLIMfit software. Imaging was performed using MPE excitation at 900 nm and a ×10 air objective with an NA of 0.30. Each FOV is 1 mm × 1 mm

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Plant Methods

ISSN: 1746-4811