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Fig. 2 | Plant Methods

Fig. 2

From: In vivo label-free mapping of the effect of a photosystem II inhibiting herbicide in plants using chlorophyll fluorescence lifetime

Fig. 2

Schematic diagram of the wide field imaging macroscope. 440 nm pulsed laser beam is passed through a cleanup filter FI and expanded using a diverging lens, L1 to illuminate a leaf. Fluorescence from the leaf is collected by a camera lens, L2, and passed through an emission filter, F2, before it reaches a high rate imager (HRI). The collected signal is imaged on to a sCMOS camera by a pair of relay lenses L3, L4. The HRI is synchronized with the excitation laser via a temporal delay generator

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Plant Methods

ISSN: 1746-4811