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Fig. 1 | Plant Methods

Fig. 1

From: An efficient protocol for perennial ryegrass mesophyll protoplast isolation and transformation, and its application on interaction study between LpNOL and LpNYC1

Fig. 1

Effects of mannitol concentration on ryegrass protoplast isolation. A Isolated protoplast on a hemocytometer; B effect of mannitol concentration on protoplast density; C effect of mannitol concentration on protoplast viability counted using the FDA staining assay. Mannitol concentration was set at 0.3, 0.4, 0.5, 0.6, or 0.7 M, respectively. The number of intact and viable protoplasts was counted visually for round and intact protoplasts under a light microscope (OLYMPUS Model BX53, Tokyo, Japan). At least 30 protoplasts were counted in one scope, and the means were from ≥3 scopes. Different letters represent statistically significant difference at p = 0.05, and bars above columns represent standard errors

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Plant Methods

ISSN: 1746-4811