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Fig. 1 | Plant Methods

Fig. 1

From: High spatio-temporal-resolution detection of chlorophyll fluorescence dynamics from a single chloroplast with confocal imaging fluorometer

Fig. 1

Principle and basic components of a confocal imaging fluorometer. Laser beam is reflected by a dichroic mirror and goes through a set of scanning mirrors, then focused by an objective lens onto the specimen. Fluorescence signals is epi-collected in the same path, and filtered out by the dichroic mirror. A confocal pinhole is used to allow only fluorescence emitted from the focal plane being detected by the PMT

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